Seahorse XFe96

Seahorse XF Analyzers measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) at intervals of approximately 5-8 minutes. OCR is an indicator of mitochondrial respiration, and ECAR is largely the result of glycolysis.

Real-time measurements of OCR and ECAR are made by isolating an extremely small volume (about 2 μL) of medium above a monolayer of cells within a microplate.

Cellular oxygen consumption (respiration) and proton excretion (glycolysis) cause rapid, easily measurable changes to the concentrations of dissolved oxygen and free protons in this "transient microchamber" which are measured every few seconds by solid state sensor probes residing 200 microns above the cell monolayer. The instrument measures the concentrations for 2-5 minutes then calculates the OCR and ECAR, respectively.

Once a measurement is completed, the probes lift which allows the larger medium volume above to mix with the medium in the transient microchamber, restoring cell values to baseline. An integrated drug delivery system allows sequential addition of up to four compounds per well at user-defined intervals.

Read more on Agilents home page.

Agilent has made these 3 small videos to show how the system works.

In case you have questions about consumables we recommend to contact the vendor on seahorse.comsumables@agilent.com

Seahorse XF Imaging and Normalization System

The Seahorse system can be used together with the Cytation 1 system. Cytation 1 is a key component of Agilent Technologies’ Seahorse XF Imaging and Normalization System. The integrated solution with Cytation 1 combines cellular analysis and imaging for an intuitive workflow to streamline XF data interpretation, the software Wave make sure to collect and calculate the data from both Seahorse and Cytation instruments.

Specification

Detail
Number of Assay Wells 92
Microchamber volume 2 µL
Best For
Phenotypic Screening
Testing many conditions at one time
Dose-response studies
Spheroids
Plate Format 96-well microplate

Feature

Compatibility with both adherent and suspension cells as well as isolated mitochondria and non-mammalian samples.

Ability to perform up to 4 independent injections per well with automatic mixing

Automatic calculation of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR).

Simultaneous measurement of OCR and ECAR in the same well

Analytical Sensitivity for small sample sizes

Label-free detection in real time
Windows-compatible desktop analysis software (Wave) for plotting, reporting, analyzing, and exporting your XF data