Resources

You may need other probes or dyes than the conventional colors that we use in flow cytometry. We have compiled a list of other colors for you inspiration.

• Fluorochrome Table Alexa Fluor Dyes
• Fluorochrome Table Brilliant Violet Dyes
• Fluorochrome Table Cell Function Probes
• Fluorochrome Table Cy Dyes
• Fluorochrome Table DyLight Dyes
• Fluorochrome Table Fluorescent Proteins
• Fluorochrome Table Nucleic Acid Probes
• Fluorochrome Table Other probes
• Fluorochrome Table Reactive and Conjugated Probes

If you are interested in using fluorescent proteins in your research, some relevant information may be found in this data base.

• https://www.fpbase.org/about/

Video tutorials by J Paul Robinson

• Excitation & emission in flow cytometry (YouTube 10:43)
• Flow cytometry & Light scatter: What is it and what does it measure? (YouTube 6:10)
• Fluidics in flow cytometry (YouTube 7:34)
• CytoFLEX technology, Fluidics systems (YouTube 4:22)

Video tutorial by Derek Davies

• This is a series of YouTube videos where you can pick the subject of interest.

Video tutorial by Andrew Filby

• Imaging flow cytometry: A brief overview (YouTube 35:52)

Useful Spectrum viewers to determine if a fluorochrome work on an instrument

• https://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer 

• http://www.biolegend.com/spectraanalyzer
• https://www.bio-rad-antibodies.com/spectraviewer.html
• https://searchlight.semrock.com/
• https://www.thermofisher.com/order/fluorescence-spectraviewer#!/

Application notes and Protocols

• http://www.bdbiosciences.com/eu/applications/c/applications

Worthington Tissue Dissociation Guide

• http://www.worthington-biochem.com/tissuedissociation/default.html

E-Learning (flow cytometry, software, and hardware theory)

• http://www.bdbiosciences.com/eu/s/training/e-learning
• http://www.lifetechnologies.com/dk/en/home/support/tutorials.html
  
• http://www.biolegend.com/webtoolstab

Webinars

• BD Biosciences, several webinars
  http://www.bdbiosciences.com/eu/s/webinars/overview
• Life technologies, several webinars
  http://www.lifetechnologies.com/dk/en/home/support/tutorials.html
• Biolegend, multicolour flow, about the Brilliant Violet fluorophores
  http://cnpg.comparenetworks.com/113777-Multicolor-Flow-Cytometry-The-Brilliant-Violet-Family-of-Fluorophores-Multicolor-Qi/
• Biolegend, multicolour flow, Modulating in vivo T-cell activation
  https://www.biolegend.com/en-us/videos/modulating-in-vivo-t-cell-activation-15-color-immunophenotyping-cytokine-analysis-and-cellular-redistribution

Panel design can be a difficult and time-consuming process but is essential to obtaining good data. Spillover spread matrix can be a useful tool to overlook fluorochrome combination that are cumbersome to work with to avoid reagent selections that may negatively affect population resolution.

On the following page we have made a graphic visualization of the spread matrix (PDF file) for each of our instruments. The data are actual measurements, and can be used to design your next antibody panel. In this way you can be sure that any panel design you make will fit with the instrument you are to use.

One of the most fundamental questions in biology is which types of cells form different tissues and organs in a functionally coordinated fashion. Larger-scale single cell sequencing and biology experiment studies are now rapidly opening up new ways to track this question by revealing substantial cell markers for distinguishing different cell types in tissues. Here, we developed the CellMarker databases, aiming to provide a comprehensive and accurate resource of cell markers for various cell types in tissues of human and mouse.