Courses

We offer several kinds of courses at the Core facility. Find detailed course descriptions below as well as introduction videos.

Internal Courses

Internal courses to get access to use our instruments. Mandatory for all new users, and existing users that need access to a new instrument.

 

 

 

 

 

 

 

 

 

 

 

 

PhD Course

Part of the Faculty's PhD programme.

 

 

 

 

 

 

 

 

 

 

 

 

 

Course title: Advanced flow cytometry II 

Learning objectives: A student who has met the objectives of the course will be able to:

  • Optimize staining of a sample and settings for the instrument before running samples
  • Design and make an instrument dependent spread matrix. Use the spread matrix for design of multicolor flow panels
  • Understand the standards of publishing and storing flow cytometry related data
  • Analyze and interpret data based on tSNE, UMAP, FlowSOM and similar software
  • Understand the principle of barcoding in flow cytometry
  • Design a multicolor experiment with relevant controls, fit for interpretation and publication

Content: The course is divided into five themes, each covering part of a flow cytometer experiment. We will take you through design of multi-color panels, how to achieve the best sensitivity on your measurements, what controls to pick for the experiment, and how to analyze/evaluate the experiment. We show you how barcoding of samples can optimize the time spend at the instruments and minimize variation of samples from different time points e.g. kinetic studies in patients.

The course includes the use of FlowLogic and FlowJo for analyzing samples, as well as tSNE, UMAP, FlowSOM and ClusterExplore for analysis and generation of hypothesis.

Participants: PhD Students and people on similar level.

We recommend that you have had our Basic course in flow cytometry and cell sorting or a similar course, and we recommend that you have worked with multicolor flow cytometry for at least a year, in order for you to get as much out of the course as possible. You will work with different instruments and software, and there will not be time to train people in basic use of an instrument and knowledge about flow cytometry.

Relevance to graduate programs: The course is relevant to PhD students from the following graduate programs at the Graduate School of Health and Medical Sciences, UCPH: Immunology and Infectious Diseases.

Language: English

Form: Each theme is a 2-week block where students have to watch some e-learning videos and read some papers to prepare for the practical part. For each theme, we will meet for 1 or 2 days to make practical and/or theoretical exercises, related to the theme.

ECTS-credits: 5.4 point

Course director: Professor Jan Pravsgaard Christensen, ISIM, 35327873, jpc@sund.ku.dk

Teachers: Jan Pravsgaard Christensen, staff, PhD, postdocs, and technicians at the department

Dates: The course covers six topics each of two weeks home studies and one full day with practical exercises. See individual postings on the Graduate School of Health and Medical Sciences.

Course location: Faculty of Health and Medical Sciences. Panum, Blegdamsvej 3B, DK-2200 Copenhagen N. Maersk Tower, 14th floor.

Next Courses: See individual postings on the Graduate School of Health and Medical Sciences website. For questions regarding course applications please contact the Graduate School of Health and Medical Sciences.

Seats to PhD students from other Danish universities will be allocated on a first-come, first-served basis and according to the applicable rules. Applications from other participants will be considered after the last day of enrolment.

 

 

 

 

 

Non PhD Courses

Courses for technicians, bio analysts, master students, postdocs, and supervisors that need introduction to flow cytometry. We offer three different courses in this category:

 

 

Course title: Basic flow cytometry and cell sorting, theoretical course

Learning objectives: A student who has met the objectives of the course will be able to:

  • The student will be able to identify, relevant protocols to analyze the samples with, define relevant controls needed for analysis, and understand the limitation of flow cytometry
  • The student will be able to plan their experimental setup for running and analyzing flow cytometry samples, analyze the achieved data, and compare the data to other groups.

Content: The course will take you through the basic knowledge of flow cytometry and cell sorting, how to design and choose the right experiment, and some of the pitfalls in flow cytometry. 

Participants: Technicians, bio analysts, master students, postdocs, and supervisors that need introduction to flow cytometry. No previous skills in flow cytometry are needed, people with previous experience can also benefit from the course.

Number of seats: No limitation, the course have continues enrolment.

Language: English

Form:
E-learning course with videos to watch and small test to evaluate yourself. The classroom will be open for 2 month after enrolment

Teachers: Jan Pravsgaard Christensen

Dates: Continuous enrolment

Course location: Where ever you have time to watch the e-learning videos

Price: 5000,- plus vat. Invoice will be issued in the month before enrolment

Registration: Registration should be done in formula below, depended on place of employment.

Registration UCPH employees        Registration non-UCPH employee

Questions to Course director:
Jan P. Christensen
Department of Immunology and Microbiology
Blegdamsvej 3c, Maersk Tower 07-13-59
Phone: 35327873
Mail: facsmailbox@sund.ku.dk

 

 

 

 

 

 

Course title: Basic flow cytometry and cell sorting, theoretical and practical course

Learning objectives: A student who has met the objectives of the course will be able to:

  • The student will be able to run simple samples/protocols on flow cytometry and cell sorter, analyze the achieved data, and compare the data to other groups, evaluate presented data from flow cytometry, and discuss flow cytometry on a basic level.

Content: The course will take you through basic knowledge of how to operate a flow cytometer or cell sorter, and how to analyse the data. Several techniques will be demonstrated and the student will get a chance to work on our different instruments (FACSCalibur, LSR-II, and Aria-II).
The course is focused on the use of diva software, and to some extent CellQuest because of our choice of instruments, but independent software such as FlowLogic will also be demonstrated.

Participants: Technicians, Bio Analysts, Master Students, Postdocs, and Supervisors that need introduction to flow cytometry. No previous skills in flow cytometry are needed. People with previous experience can also benefit from the course.

Number of seats: There will be 4 seats on each course that run at the same time as our PhD courses, typically January, February, September, and October each year. Scheduling will be agreed upon when signing up for the course.

Language: English

Form:
Theoretical part: E-learning course with videos to watch and small test to evaluate yourself.

Practical part:

  • One day (12-16) with practical exercises on the instruments (Calibur, LSR-II, and ARIA-II)
  • One day (9-16) with practical exercises on the instruments (Calibur, LSR-II, and ARIA-II)
  • One day (4-5 hours) with data analysis, test and evaluation

Teachers: Jan Pravsgaard Christensen, Liselotte Norup, Lau Larsen, and Rasmus Nielsen.

Course location:

  • Theoretical part: Watch the e-learning videos anywhere you want.
  • Practical part: Faculty of Health and Medical Sciences, Blegdamsvej 3c, 14th floor, DK-2200 Copenhagen N.

Price: 9000,- plus vat. Invoice will be issued in the month before the course

Registration: Registration should be done in the formula below, dependent on place of employment.

Registration UCPH employee        Registration non-UCPH employee

Questions to Course director:
Jan P. Christensen
Department of Immunology and Microbiology
Blegdamsvej 3c, Maersk Tower 07-13-59
Phone: 35327873
Mail: facsmailbox@sund.ku.dk

 

Course title: Advanced flow cytometry, theoretical and practical course

Learning objectives: A student who has met the objectives of the course will be able to:

  • Optimize staining of a sample and settings for the instrument before running samples
  • Design and make an instrument dependent spread matrix. Use the spread matrix for design of multicolor flow panels
  • Understand the standards of publishing and storing flow cytometry related data
  • Analyze and interpret data based on tSNE, UMAP, FlowSOM and similar software
  • Understand the principle of barcoding in flow cytometry
  • Design a multicolor experiment with relevant controls, fit for interpretation and publication

Content: The course is divided into five themes, each covering part of a flow cytometer experiment. We will take you through design of multi-color panels, how to achieve the best sensitivity on your measurements, what controls to pick for the experiment, and how to analyze/evaluate the experiment. We show you how barcoding of samples can optimize the time spend at the instruments and minimize variation of samples from different time points e.g. kinetic studies in patients.

The course includes the use of FlowLogic and FlowJo for analyzing samples, as well as tSNE, UMAP, FlowSOM and ClusterExplore for analysis and generation of hypothesis.

Participants: Technicians, Bio Analysts, Master Students, Postdocs, and Supervisors that need introduction to flow cytometry.

We recommend that you have had our Basic course in flow cytometry and cell sorting or a similar course, and we recommend that you have worked with multicolor flow cytometry for at least a year, in order for you to get as much out of the course as possible. You will work with different instruments and software, and there will not be time to train people in basic use of an instrument and knowledge about flow cytometry.

Number of seats: There will be 4 seats on each course that run at the same time as our PhD courses, typically February-April, once every two weeks with activity in the facility.

Language: English

Form:
Each theme is a 2-week block where students have to watch some e-learning videos and read some papers to prepare for the practical part. For each theme, we will meet for 1 or 2 days to make practical and/or theoretical exercises, related to the theme.

Teachers: Jan Pravsgaard Christensen, Liselotte Norup, Lau Larsen and Rasmus Nielsen.

Course location:

  • Theoretical part: Watch the e-learning videos anywhere you want.
  • Practical part: Faculty of Health and Medical Sciences, Blegdamsvej 3c, 14th floor, DK-2200 Copenhagen N.

Price: 9000,- plus vat. Invoice will be issued in the month before the course

Registration: Registration should be done in the formula below, dependent on place of employment.

Registration UCPH employee        Registration non-UCPH employee

Questions to Course director:
Jan P. Christensen
Department of Immunology and Microbiology
Blegdamsvej 3c, Maersk Tower 07-13-59
Phone: 35327873
Mail: facsmailbox@sund.ku.dk

 

 

 

 

Introduction videos

Get a sense of the content of the courses.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Upcoming PhD Courses

The basic course are typical in January, February, September, and October.

Look at the Graduate School of Health and Medical Sciences' homepage to sign up. The courses are in high demand so make sure to check when the next course comes online.

If you have questions regarding how to sign up, please contact the Graduate School of Health and Medical Sciences.