Cytation 1
Cytation 1 was acquired to support the Seahorse instrument; here it can normalize data to account for different number of cells pr. well, making data more comparable, but it can do many more things.
So far the instrument can only do Hoechst or DAPI based cell counting. If you are in need of other assays we would like to hear from you and then we will consider which extra filter to buy.
BioTek’s unique approach to imaging and microscopy requirements offers a range of cell imaging systems designed to suit many applications from simple, fixed cell assays and slide scanning to advanced, environmentally controlled long term live cell kinetic assays and 3D spheroid formation imaging. Here are some examples, the list is longer, of what the system can be used for.
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Apoptosis |
Cell Counting |
Cell Counting: Label-Free |
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Cell Migration/Invasion |
Cell Proliferation |
Cell Viability/Toxicity |
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Confluence |
Immunofluorescence |
Live Cell Imaging |
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Microbiology |
Phenotypic Assays |
Transfection Efficiency |
Specifications
In bold is the current option on our instrument.
GENERAL |
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|---|---|
| Detection modes | UV-Vis absorbance Fluorescence intensity Luminescence Fluorescence polarization Time-resolved fluorescence |
| Microplate types | Monochromator: 6- to 384-well plates Filters: 6- to 1536-well plates Imaging: 6- to 1536-well plates |
| Temperature control | 4-Zone incubation to 45 °C with Condensation Control™; Variation ±0.2 °C at 37 °C |
IMAGING SYSTEM |
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| Imaging mode | Fluorescence and high contrast brightfield (2.5x Zeiss, 4x, 10x, 20x, 40x and 60x) |
| Imaging method | Single color, multicolor, montage, time lapse, z-stacking |
| Camera | 16-bit gray scale, Sony CCD |
| Imaging filter cubes available | DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP, Alexa568, Ex377 / Em647 |
| Imaging LED cubes available | 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm |
| Image collection rate | Image-based autofocus: 96 wells, 1 color (DAPI), 4x, 6 minutes 96 wells, 3 colors, 4x, 12 minutes Laser autofocus: 96 wells, 1 color (DAPI), 4x, <3 minutes 96 wells, 3 colors, 4x, <7 minutes, 30 seconds Burst Mode: 10 fps, single well, single color at <= 50ms integration time |
FLUORESCENCE INTENSITY |
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| Light source | Xenon flash |
| Wavelength range | Filters: 200 - 700 nm |
| Dynamic range | 7 decades |
| Sensitivity | Fluorescein: 0.25 pM (0.025 fmol/well, 384-well plate) |
| Reading speed (kinetic) | 96 wells: 11 seconds 384 wells: 22 seconds |
LUMINESCENCE |
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| Wavelength range | 300 - 700 nm |
| Dynamic range | >6 decades |
| Sensitivity | 10 amol ATP (flash) 100 amol (glow) |