Invitrogen Qubit 4 Fluorometer
The Qubit 4 instrument provides and easy, fast and relatively accurate way of determining DNA and RNA concentrations by detection of fluorescent dyes, which bind specifically to the target of interest. The Qubit is not as accurate for NGS library quantification as Real-Time PCR, as it detects all dsDNA in the sample and not only the dsDNA that is sequenceable. However, if you are doing standard small whole genome library preparations etc. for sequencing on the Miseq or need to measure library input conc. it is a budget-friendly alternative to Real-Time PCR.
Specifications
| Sample vol./measurement | 1-20 µL |
|---|---|
| Sample throughput | 1 sample at a time |
| Runtime | 3 sec./sample |
| Emission filters | Green (510-580 nm) and Yellow (665-720 nm) |
| Excitation filters | Blue (430-495 nm) and red (600-645 nm) |
| LED light source | Blue (max: 470 nm) red (max: 635 nm) |
| Photodiode detector range | 300-1000 nm |
Kits
There are several kits available for the Qubit. As a service, the Core facility will sell reagents and consumables for the 1X dsDNA HS Assay, which is designed to be used with the initial sample conc. of 10 pg/µL to 100 ng/ µL