10X Visium CytAssist
The Visium CytAssist is designed to simplify the Visium FFPE workflow by facilitating the transfer of transcriptomic probes from standard glass slides to Visium slides, enabling a simpler and more precise placement of the tissue section relative to the Visium slide. Furthermore, it enables capturing a larger area of the given tissue sample (11x11mm) in comparison to the manual Visium FFPE workflow (6.5x6.5mm).
The Visium CytAssist can be used with samples archived in FFPE blocks or pre-sectioned tissue on standard glass slides. Note that the Visium CytAssist workflow is only compatible with human or mouse tissue and specifically requires the Visium CytAssist Spatial Gene Expression for FFPE kits.
| Min. slide dimensions | 24.8 x 74.4mm |
|---|---|
| Max. slide dimensions | 25.3 x 76.2mm |
| Number of samples/Visium slide | 2 |
| Capture area size | 6.5x6.5mm/11x11mm |
| Run time | App. 30 min. |
Important notes on tissue placement
Tissue sections need to be placed a minimum of 5 mm from the long side of the glass slide and 15 mm from the short sides, to ensure a successful transfer of analytes to the Visium CytAssist Spatial Gene expression slide.
If the glass slides contain frosted parts, letters or markings the 15mm distance is measured here from, please see diagram below.
Additional information on the Visium CytAssist Spatial Gene Expression for FFPE can be found here:
https://www.10xgenomics.com/support/spatial-gene-expression-ffpe/documentation