Fortessa X20 5 laser

The Fortessa X20 instrument is the new version of Fortessa (5 laser) but with a newer technology and detection system. The X20 is similar to the Fortessa 5 laser system with lasers and filters.

Specification

Lasers

  • 488 nm laser (50 mW)
  • 640 nm laser (40 mW)
  • 405 nm laser (50 mW)
  • 561 nm laser (50 mW)
  • 355 nm laser (20 mW)

Optical system

  • Detection of 18 FL-parameters
  • Detection of forward scatter light (FSC) with photo diode
  • Direction of side scatter light (SSC) and fluorescence signal towards PMT arrays with optical fibers.

Blue laser detection (488 nm)*

  • 505 LP, 530/30 nm FITC
  • 685 LP, 710/50 nm PerCP

Red laser detection (640 nm)*

  • 670/30 nm APC
  • 690 LP, 730/45 nm Alexa flour 700
  • 750 LP, 780/60 nm APC-Cy7

Violet laser detection (405 nm)* 

  • 431/28 nm BV421
  • 505 LP, 525/50 nm BV480, BV510
  • 595 LP, 610/20 nm BV605
  • 630 LP, 670/30 nm BV650
  • 690 LP, 710/50 nm BV711
  • 750 LP, 780/60 nm BV786

Yellow/green laser detection (561 nm)* 

  • 586/15 nm PE, Tomato red
  • 600 LP, 610/20 nm PE-CF594, PI, mCherry
  • 635 LP, 670/30 nm PE-Cy5.5, 7AAD
  • 750 LP, 780/60 nm PE-Cy7

UV laser detection (355 nm)* 

  • 379/28 nm BUV395
  • 450 LP, 515/30 nm BUV496, DAPI, Hoechst
  • 690 LP, 740/35 nm BUV737

We do have additional bandpass filters that, on request, can be used in the instrument. 
Check the reference guide including a guide to setting up the color combination or use the more comprehensive list of available fluorochromes.

* Each color is just an example, you should ensure that you are using the right colors by checking the filter setting.

High Throughput Option 

  • The High Throughput Sampler (HTS) option is installed to increase productivity. It supports standard 96-well U-, V-, and flat-bottom, and 384-well flat-bottom plates.