FACS Fortessa (5 laser)

The FACS Fortessa (5 laser) is similar to the 3 laser system but have an UV, ans a yellow/green laser. The software for this instrument has an extra feature to control the UV laser and training is needed, also for experienced users, before you can use the instrument.

Specification

Lasers

  • 488 nm laser (50 mW)
  • 640 nm laser (40 mW)
  • 405 nm laser (50 mW)
  • 561 nm laser (50 mW)
  • 355 nm laser (20 mW)

Optical system

  • Detection of 18 FL-parameters
  • Detection of forward scatter light (FSC) with photo diode
  • Direction of side scatter light (SSC) and fluorescence signal towards PMT arrays with optical fibers.

Blue laser detection (488 nm)*

  • 505 LP, 530/30 nm FITC
  • 685 LP, 710/50 nm PerCP

Red laser detection (640 nm)*

  • 670/14 nm APC
  • 690 LP, 730/45 nm Alexa flour 700
  • 750 LP, 780/60 nm APC-Cy7

Violet laser detection (405 nm)* 

  • 431/28 nm BV421
  • 505 LP, 525/50 nm BV480, BV510
  • 595 LP, 610/20 nm BV605
  • 630 LP, 660/20 nm BV650
  • 690 LP, 710/50 nm BV711
  • 750 LP, 780/60 nm BV786

Yellow/green laser detection (561 nm)* 

  • 586/15 nm PE, Tomato red
  • 600 LP, 610/20 nm PE-CF594, PI, mCherry
  • 635 LP, 670/30 nm PE-Cy5.5, 7AAD
  • 750 LP, 780/60 nm PE-Cy7

UV laser detection (355 nm)* 

  • 379/28 nm BUV395
  • 450 LP, 515/30 nm BUV496, DAPI, Hoechst
  • 690 LP, 740/35 nm BUV737

We do have additional bandpass filters that, on request, can be used in the instrument. 
Check the reference guide including a guide to setting up the color combination or use the more comprehensive list of available fluorochromes.

* Each color is just an example, you should ensure that you are using the right colors by checking the filter setting.

High Throughput Option 

  • The High Throughput Sampler (HTS) option is installed to increase productivity. It supports standard 96-well U-, V-, and flat-bottom, and 384-well flat-bottom plates.