FACS Fortessa (3 laser)

The instrument is an extended version of the LSR-II and can analyze even more colors. The software and philosophy behind this instrument is somewhat different from the Calibur and training is needed, also for experienced users, before you can use the instrument.

Specification (updated 1/8-2014)

Lasers

  • Coherent Sapphire 488 nm solid-state diode laser (50 mW)
  • 640 nm JDS Uniphase HeNe, air cooled laser (40 mW)
  • 405 nm Coherent Violet laser (50 mW)

Optical system

  • Detection of 14 FL-parameters
  • Detection of forward scatter light (FSC) with photo diode
  • Direction of side scatter light (SSC) and fluorescence signal towards PMT arrays with optical fibers.

Blue laser detection (488 nm)*

  • 505 LP, 530/30 nm FITC
  • 550 LP, 575/26 nm PE
  • 600 LP, 610/20 nm PE-Texas red
  • 685 LP, 695/40 nm PE-Cy5.5
  • 750 LP, 780/60 nm PE-Cy7

Red laser detection (640 nm)*

  • 670/14 nm APC
  • 710 LP, 730/45 nm Alexa flour 700
  • 750 LP, 780/60 nm APC-Cy7

Violet laser detection (405 nm)* 

  • 431/28 nm BV421
  • 475 LP, 525/50 nm BV480 and BV510
  • 595 LP, 610/20 nm BV605
  • 630 LP, 660/20 nm BV650
  • 690 LP, 720/20 nm BV711
  • 750 LP, 800/50 nm BV786

We do have additional bandpass filters that, on request, can be used in the instrument. 
Check the reference guide including a guide to setting up the color combination or use the more comprehensive list of available fluorochromes.

* Each color is just an example, you should ensure that you are using the right colors by checking the filter setting.

High Throughput Option 

  • The High Throughput Sampler (HTS) option is installed to increase productivity. It supports standard 96-well U-, V-, and flat-bottom, and 384-well flat-bottom plates.