FACSymphony A5 6 laser

The FACSymphony A5 is the must high-end flow cytometer in the facility. The FACSymphony A5 is running under DIVA but a newer version, , and has a similar filter configuration to the FACSymphony S6 cell sorter.

Specification

Lasers

  • 355 nm laser (100 mW)
  • 405 nm laser (200 mW)
  • 488 nm laser (200 mW)
  • 561 nm laser (200 mW)
  • 637 nm laser (200 mW)
  • 785 nm laser (100 mW)

Optical system

  • Detection of 32 FL-parameters
  • Detection of forward scatter light (FSC) with photo diode
  • Direction of side scatter light (SSC) and fluorescence signal towards PMT arrays with optical fibers.

UV laser detection (355 nm)* 

  • 370 LP, 379/28 nm BUV395
  • 410 LP, 450/50 nm DAPI, Hoechst
  • 490 LP, 515/30 nm BUV496
  • 550 LP, 580/20 nm BUV563
  • 600 LP, 610/20 nm BUV615
  • 630 LP, 670/20 nm BUV661
  • 690 LP, 735/30 nm BUV737
  • 770 LP, 810/40 nm BUV805#

Violet laser detection (405 nm)* 

  • 410 LP, 431/28 nm BV421, Pacific Blue
  • 505 LP, 525/50 nm BV480, BV510
  • 550 LP, 586/15 nm BV570
  • 595 LP, 605/40 nm BV605
  • 635 LP, 677/20 nm BV650
  • 685 LP, 710/50 nm BV711
  • 735 LP, 750/30 nm BV750
  • 770 LP, 810/40 nm BV786#

Blue laser detection (488 nm)*

  • 505 LP, 530/30 nm BB515, FITC
  • 600 LP, 610/20 nm BB630-P2
  • 635 LP, 670/30 nm BB660-P2
  • 685 LP, 710/50 nm BB700, PerCP
  • 735 LP, 750/30 nm BB755-P
  • 770 LP, 810/40 nm BB790-P#

Yellow/green laser detection (561 nm)* 

  • 570 LP, 586/15 nm PE/BYG584-P, PE, Tomato red
  • 600 LP, 610/20 nm PE-CF594, PI, mCherry
  • 635 LP, 670/30 nm PE-Cy5, 7AAD
  • 690 LP, 710/50 nm PE-Cy5.5
  • 750 LP, 780/60 nm PE-Cy7#

Red laser detection (640 nm)*

  • 655 LP, 670/30 nm APC
  • 690 LP, 730/45 nm AF700/R718, Alexa flour 700
  • 750 LP, 780/60 nm APC-Cy7#

InfraRed laser detection (785 nm)* 

# NOTICE: if you use a IR color then BUV805, BV786, BB790-P, PE-Cy7, and APC-Cy7 can NOT be used due to overlap.

  • 800/12 nm IR #1
  • 820 LP, 820/60 nm IR #2

Check the reference guide including a guide to setting up the color combination or use the more comprehensive list of available fluorochromes. You can also take a look at our overview of filters on each instrument.

* Each color is just an example, you should ensure that you are using the right colors by checking the filter setting.

High Throughput Option 

  • The High Throughput Sampler (HTS) option is installed to increase productivity. It supports standard 96-well and 384-well bottom plates.