FACSVerse
The FACSVerse is our newest analysis instrument and could be the replacement for the Calibur in the future, and a substitution for the LSR-II. The software (BD FACSuite™ software) and philosophy behind this instrument is a hybrid between the Calibur and LSR II so training is needed, also for experienced users, before you can use the instrument.
Specification
Lasers
- 405 nm, 44mW
- 488 nm, 20mW
- 640 nm, 40mW
Optical system
- Detection of 8 FL-parameters
- Detection of forward scatter light (FSC) with photo diode
- Direction of side scatter light (SSC) and fluorescence signal towards PMTs
Blue laser detection (488 nm)*
- 507 LP, 527/32 nm FITC
- 560 LP, 568/42 nm PE
- 665 LP, 700/54 nm PerCP-Cy5.5
- 752 LP, 783/56 nm PE-Cy7
Red laser detection (640 nm)*
- 660/10, 660/10 nm APC
- 752 LP, 783/56 nm APC-Cy7
Violet laser detection (405 nm)*
- 448/45 nm BV421
- 500LP, 528/45 nm BV510
We do have additional bandpass filters that, on request, can be used in the instrument.
Check the reference guide including a guide to setting up the color combination or use the more comprehensive list of available fluorochromes.
* Each color is just an example, you should ensure that you are using the right colors by checking the filter setting.
Single cell collection
The FACSVerse is like the Calibur in that you can only run individual samples.