Courses
We offer several kinds of courses at the Core facility. Find detailed course descriptions below as well as introduction videos.
Internal Courses
Internal courses to get access to use our instruments. Mandatory for all new users, and existing users that need access to a new instrument.
Aim: Internal courses introduces you to a specific instrument in the facility and is mandatory before you can start working on your own in the facility. Courses are taught by CFFC staff only.
Content: A course consist of at least two sessions.
First session is a 2-3 hours introduction to the instrument.
We do NOT teach you basic flowcytometry. You need a basic knowledge of flowcytometry before using our instruments.
At the second session you perform your first experiment under supervision of CFFC staff.
We supply the cells for the first session. For following sessions you must supply your own. Use strong markers to ease the work of CFFC staff, Contact us before the session if necessary. It is not necessary to include markers from your project, and you do not need to make complex multi color stains, 3 to 4 colors are sufficient. Focus on getting a good stain of your cells to demonstrate the instrument.
If adequate overview of the instrument has not been achieved you may need additional sessions or be accompanied by a guide when using the instrument.
Staff is not necessarily familiar with all cell types and stains. Contact us to get the best sample for your training.
Instrument training
We offer an instrument course for new users of the facility. This course is mandatory before you can start using the instruments, even if you are experienced user. The training continues till sufficient skills have be acquired. If you have previous experience using similar instruments as the core facility is offering, the training will probably only be one session.
When: Instrument training courses primarily run every Tuesday.
Send a mail to us to register. Inform us on preferred date and instrument.
To attend a course you need to be registered as a user of the facility - Sign up for login here.
PhD Course
Part of the Faculty's PhD programme.
Course title: Basic flow cytometry and cell sorting I
Learning objectives: A student who has met the objectives of the course will be able to:
- The student will be able to identify, relevant protocols to analyze the samples with, define relevant controls needed for analysis, understand the limitation of flow cytometry, and be able to analyze obtained data in terms of publication/scientific presentation.
- The student will be able to plan their experimental setup for running and analyzing flow cytometry samples, apply this in a practical setting, analyze the achieved data, and compare the data to other groups.
- The student will be able to run simple samples/protocols on flow cytometry and cell sorter, evaluate presented data from flow cytometry, and discuss flow cytometry on a basic level.
Content: The course will take you through basic knowledge of how to design and choose the right experiment, how to operate the instrument, and some of the pitfalls in flow cytometry. Several techniques will be demonstrated and the student will get a chance to work with these on our different instruments (FACSCalibur, LSR-II, and Aria-II).
The course is focused on the use of diva software, and to some extent CellQuest because of our choice of instruments, but independent software such as FlowLogic will also be demonstrated.
During the course, some exercises will require preparation from day to day, since presentation / discussion with the group is needed.
Participants: PhD students and people on similar level, no previous skills in flow cytometry are needed.
Relevance to graduate programs: The course is relevant to PhD students from the following graduate programs at the Graduate School of Health and Medical Sciences, UCPH: Immunology and Infectious Diseases
Language: English
Form: E-learning lectures for two weeks and then practical and theoretical exercises for 3 days. The course has a follow up where students can get help designing their own experiments.
ECTS-credits: 5.1 point
Course director: Professor Jan Pravsgaard Christensen, ISIM, 35327873, jpc@sund.ku.dk
Teachers: Jan Pravsgaard Christensen, staff, PhD, postdocs, and technicians at the department
Dates: For the first two weeks you are expected to watch e-learning videos at home, and test you self, app 30 hours. For the next three days it is practical exercise in core facility. Se individual postings on the PhD schools homepage.
Course location: Faculty of Health and Medical Science, Panum, Blegdamsvej 3B, DK-2200 Copenhagen N. Maersk Tower, 14th floor.
Next Courses: See individual postings on the Graduate School of Health and Medical Sciences website. For questions regarding course application please contact the Graduate School of Health and Medical Sciences.
Seats for PhD Students from other Danish Universities will be allocated on a first-come, first-served basis and according to the applicable rules. Applications from other participants will be considered after the last day of enrolment.
Course title: Advanced flow cytometry II
Learning objectives: A student who has met the objectives of the course will be able to:
- Optimize staining of a sample and settings for the instrument before running samples
- Design and make an instrument dependent spread matrix. Use the spread matrix for design of multicolor flow panels
- Understand the standards of publishing and storing flow cytometry related data
- Analyze and interpret data based on tSNE, UMAP, FlowSOM and similar software
- Understand the principle of barcoding in flow cytometry
- Design a multicolor experiment with relevant controls, fit for interpretation and publication
Content: The course is divided into five themes, each covering part of a flow cytometer experiment. We will take you through design of multi-color panels, how to achieve the best sensitivity on your measurements, what controls to pick for the experiment, and how to analyze/evaluate the experiment. We show you how barcoding of samples can optimize the time spend at the instruments and minimize variation of samples from different time points e.g. kinetic studies in patients.
The course includes the use of FlowLogic and FlowJo for analyzing samples, as well as tSNE, UMAP, FlowSOM and ClusterExplore for analysis and generation of hypothesis.
Participants: PhD Students and people on similar level.
We recommend that you have had our Basic course in flow cytometry and cell sorting or a similar course, and we recommend that you have worked with multicolor flow cytometry for at least a year, in order for you to get as much out of the course as possible. You will work with different instruments and software, and there will not be time to train people in basic use of an instrument and knowledge about flow cytometry.
Relevance to graduate programs: The course is relevant to PhD students from the following graduate programs at the Graduate School of Health and Medical Sciences, UCPH: Immunology and Infectious Diseases.
Language: English
Form: Each theme is a 2-week block where students have to watch some e-learning videos and read some papers to prepare for the practical part. For each theme, we will meet for 1 or 2 days to make practical and/or theoretical exercises, related to the theme.
ECTS-credits: 5.4 point
Course director: Professor Jan Pravsgaard Christensen, ISIM, 35327873, jpc@sund.ku.dk
Teachers: Jan Pravsgaard Christensen, staff, PhD, postdocs, and technicians at the department
Dates: The course covers six topics each of two weeks home studies and one full day with practical exercises. See individual postings on the Graduate School of Health and Medical Sciences.
Course location: Faculty of Health and Medical Sciences. Panum, Blegdamsvej 3B, DK-2200 Copenhagen N. Maersk Tower, 14th floor.
Next Courses: See individual postings on the Graduate School of Health and Medical Sciences website. For questions regarding course applications please contact the Graduate School of Health and Medical Sciences.
Seats to PhD students from other Danish universities will be allocated on a first-come, first-served basis and according to the applicable rules. Applications from other participants will be considered after the last day of enrolment.
Non PhD Courses
Courses for technicians, bio analysts, master students, postdocs, and supervisors that need introduction to flow cytometry. We offer three different courses in this category:
Course title: Basic flow cytometry and cell sorting, theoretical course
Learning objectives: A student who has met the objectives of the course will be able to:
- The student will be able to identify, relevant protocols to analyze the samples with, define relevant controls needed for analysis, and understand the limitation of flow cytometry
- The student will be able to plan their experimental setup for running and analyzing flow cytometry samples, analyze the achieved data, and compare the data to other groups.
Content: The course will take you through the basic knowledge of flow cytometry and cell sorting, how to design and choose the right experiment, and some of the pitfalls in flow cytometry.
Participants: Technicians, bio analysts, master students, postdocs, and supervisors that need introduction to flow cytometry. No previous skills in flow cytometry are needed, people with previous experience can also benefit from the course.
Number of seats: No limitation, the course have continues enrolment.
Language: English
Form:
E-learning course with videos to watch and small test to evaluate yourself. The classroom will be open for 2 month after enrolment
Teachers: Jan Pravsgaard Christensen
Dates: Continuous enrolment
Course location: Where ever you have time to watch the e-learning videos
Price: 6.000,- plus vat. Invoice will be issued in the month before enrolment
Registration: Registration should be done in formula below, depended on place of employment.
Registration UCPH employees Registration non-UCPH employee
Questions to Course director:
Jan P. Christensen
Department of Immunology and Microbiology
Blegdamsvej 3c, Maersk Tower 07-13-59
Phone: 35327873
Mail: facsmailbox@sund.ku.dk
Course title: Basic flow cytometry and cell sorting, theoretical and practical course
Learning objectives: A student who has met the objectives of the course will be able to:
- The student will be able to run simple samples/protocols on flow cytometry and cell sorter, analyze the achieved data, and compare the data to other groups, evaluate presented data from flow cytometry, and discuss flow cytometry on a basic level.
Content: The course will take you through basic knowledge of how to operate a flow cytometer or cell sorter, and how to analyse the data. Several techniques will be demonstrated and the student will get a chance to work on our different instruments (FACSCalibur, LSR-II, and Aria-II).
The course is focused on the use of diva software, and to some extent CellQuest because of our choice of instruments, but independent software such as FlowLogic will also be demonstrated.
Participants: Technicians, Bio Analysts, Master Students, Postdocs, and Supervisors that need introduction to flow cytometry. No previous skills in flow cytometry are needed. People with previous experience can also benefit from the course.
Number of seats: There will be 4 seats on each course that run at the same time as our PhD courses, typically January, February, September, and October each year. Scheduling will be agreed upon when signing up for the course.
Language: English
Form:
Theoretical part: E-learning course with videos to watch and small test to evaluate yourself.
Practical part:
- One day (12-16) with practical exercises on the instruments (Calibur, LSR-II, and ARIA-II)
- One day (9-16) with practical exercises on the instruments (Calibur, LSR-II, and ARIA-II)
- One day (4-5 hours) with data analysis, test and evaluation
Teachers: Jan Pravsgaard Christensen, Liselotte Norup, Lau Larsen, and Rasmus Nielsen.
Course location:
- Theoretical part: Watch the e-learning videos anywhere you want.
- Practical part: Faculty of Health and Medical Sciences, Blegdamsvej 3c, 14th floor, DK-2200 Copenhagen N.
Price: 10.000,- plus vat. Invoice will be issued in the month before the course
Registration: Registration should be done in the formula below, dependent on place of employment.
Registration UCPH employee Registration non-UCPH employee
Questions to Course director:
Jan P. Christensen
Department of Immunology and Microbiology
Blegdamsvej 3c, Maersk Tower 07-13-59
Phone: 35327873
Mail: facsmailbox@sund.ku.dk
Course title: Advanced flow cytometry, theoretical and practical course
Learning objectives: A student who has met the objectives of the course will be able to:
- Optimize staining of a sample and settings for the instrument before running samples
- Design and make an instrument dependent spread matrix. Use the spread matrix for design of multicolor flow panels
- Understand the standards of publishing and storing flow cytometry related data
- Analyze and interpret data based on tSNE, UMAP, FlowSOM and similar software
- Understand the principle of barcoding in flow cytometry
- Design a multicolor experiment with relevant controls, fit for interpretation and publication
Content: The course is divided into five themes, each covering part of a flow cytometer experiment. We will take you through design of multi-color panels, how to achieve the best sensitivity on your measurements, what controls to pick for the experiment, and how to analyze/evaluate the experiment. We show you how barcoding of samples can optimize the time spend at the instruments and minimize variation of samples from different time points e.g. kinetic studies in patients.
The course includes the use of FlowLogic and FlowJo for analyzing samples, as well as tSNE, UMAP, FlowSOM and ClusterExplore for analysis and generation of hypothesis.
Participants: Technicians, Bio Analysts, Master Students, Postdocs, and Supervisors that need introduction to flow cytometry.
We recommend that you have had our Basic course in flow cytometry and cell sorting or a similar course, and we recommend that you have worked with multicolor flow cytometry for at least a year, in order for you to get as much out of the course as possible. You will work with different instruments and software, and there will not be time to train people in basic use of an instrument and knowledge about flow cytometry.
Number of seats: There will be 4 seats on each course that run at the same time as our PhD courses, typically February-April, once every two weeks with activity in the facility.
Language: English
Form:
Each theme is a 2-week block where students have to watch some e-learning videos and read some papers to prepare for the practical part. For each theme, we will meet for 1 or 2 days to make practical and/or theoretical exercises, related to the theme.
Teachers: Jan Pravsgaard Christensen, Liselotte Norup, Lau Larsen and Rasmus Nielsen.
Course location:
- Theoretical part: Watch the e-learning videos anywhere you want.
- Practical part: Faculty of Health and Medical Sciences, Blegdamsvej 3c, 14th floor, DK-2200 Copenhagen N.
Price: 10.000,- plus vat. Invoice will be issued in the month before the course
Registration: Registration should be done in the formula below, dependent on place of employment.
Registration UCPH employee Registration non-UCPH employee
Questions to Course director:
Jan P. Christensen
Department of Immunology and Microbiology
Blegdamsvej 3c, Maersk Tower 07-13-59
Phone: 35327873
Mail: facsmailbox@sund.ku.dk
Introduction videos
Get a sense of the content of the courses.
Upcoming PhD Courses
The basic course are typical in January, February, September, and October.
Look at the Graduate School of Health and Medical Sciences' homepage to sign up. The courses are in high demand so make sure to check when the next course comes online.
If you have questions regarding how to sign up, please contact the Graduate School of Health and Medical Sciences.